Journal: Pathogens
Article Title: Wolbachia Promotes an Anti-Angiogenic Response Using an In Vitro Model of Vascular Endothelial Cells in Relation to Heartworm Disease
doi: 10.3390/pathogens13070603
Figure Lengend Snippet: Effects of rWSP on VEGF-A, VEGFR-1/sFlt1, VEGFR-2, sEndoglin, and mEndoglin production in unstimulated cultures ( ● ) and cultures stimulated with VEGF-A ( ● ), rWSP ( ● ), and rWSP + VEGF-A ( ● ). Results are expressed as the mean ± SEM of 3 independent experiments. The asterisk (*) and the cross (+) indicate significant differences ( p < 0.05) (rWSP: Recombinant Wolbachia Mayor Surface Protein; VEGF-A: Vascular Endothelial Grown Factor-A; VEGFR1/sFlt1; soluble Vascular Endothelial Grown Factor Receptor 1; VEGFR-2: soluble Vascular Endothelial Grown Factor Receptor 2; sEndoglin: soluble eEndoglin; mEndoglin: membrane Endoglin).
Article Snippet: VEGF-A, soluble VEGFR-1 (VEGFR-1/sFlt1), VEGFR-2, and soluble Endoglin (sEndoglin) concentrations were measured in the supernatants, and membrane Endoglin (mEndoglin) concentration was measured in the cell listed in HUVEC-stimulated and unstimulated cultures for the first 24 h by ELISA using a Human VEGF-A, VEGFR-1/sFlt1, VEGFR-2, and Endoglin Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions.
Techniques: Recombinant, Membrane